Peer-reviewed veterinary case report
Testing a new rapid blood test for diagnosing Brucella canis in dogs
By Kim, Jong Wan et al.·Published in The Journal of veterinary medical science·2007·National Veterinary Research and Quarantine Service, South Korea·View original on PubMed →
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Original publication title: Evaluation of immunochromatographic assay for serodiagnosis of Brucella canis.
- Species:
- dog
Plain-English summary
A group of dogs suspected of having brucellosis (a contagious disease that can cause late-term abortions in females and reproductive issues in males) were tested using a new rapid test called the immunochromatographic assay (ICA). This test was found to be as accurate as traditional methods for diagnosing the disease. In a study, nearly 40% of dogs from various breeds tested positive for brucellosis using the ICA. The results suggest that the ICA is a reliable and quick way for veterinarians to diagnose this serious condition in dogs, helping them get the right treatment faster.
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Abstract
Canine brucellosis is a contagious disease with venereal and oral modes of transmission that produces late abortion in females, epididymides and prostates in males. Diagnosis is difficult because of unstable serum antibody titers that vary from individual to individual as well as between different methods used for their detection. The objective of this work was to evaluate the clinical utility of the immunochromatographic assay (ICA) for serodiagnosis of dogs suspected of having brucellosis, and results were compared with those obtained for hemoculture (HC) and the rapid screening agglutination with 2-mercaptoethanol (2-ME RSAT). The all experimentally infected dogs were positive in ICA, HC and 2-ME RSAT from 5 weeks, 7 weeks, and 3 weeks after infection, respectively. Also, among dogs selected from 10 different breed kennels occurred brucellosis, 24.8%, 39.5% and 39.1% of dogs tested were detected as positive with HC, 2-ME RSAT and ICA, respectively. The kappa value between 2-ME RSAT and ICA was 0.89. The results of this study showed that sensitivity and specificity of the ICA are comparable with those obtained using conventional serological and bacteriological test for brucellosis. In conclusion, the ICA kit provides a handy and accurate tool for the rapid serodiagnosis of canine brucellosis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/18057823/