Peer-reviewed veterinary case report
How Wright-stained urine tests find bacteria in dogs
By Swenson, Cheryl L et al.·Published in Journal of the American Veterinary Medical Association·2004·Department of Pathobiology and Diagnostic Investigation, United States·View original on PubMed →
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Original publication title: Evaluation of modified Wright-staining of urine sediment as a method for accurate detection of bacteriuria in dogs.
- Species:
- dog
Plain-English summary
A study found that using a special staining method on urine samples from dogs improved the detection of bacteria in their urine. This method, called modified Wright-staining, was compared to the standard way of examining urine and showed better results in identifying infections. Out of 459 urine samples tested, the Wright-stained method was more accurate, helping vets diagnose urinary tract infections more effectively. This means that if your dog is showing signs of a urinary infection, like frequent urination or straining to urinate, this new testing method could help your vet find the problem faster and more reliably.
People also search for: dog urinary tract infection symptoms · how to test dog urine for bacteria · modified Wright stain dog urine test
Abstract
OBJECTIVE: To compare the findings of light microscopic evaluation of routine unstained wet-mounted preparations and air-dried, modified Wright-stained preparations of urine sediment with results of quantitative aerobic bacteriologic culture of urine. DESIGN: Masked prospective study. SAMPLE POPULATION: 459 urine samples collected by cystocentesis from 441 dogs. PROCEDURE: Urinalyses and quantitative bacteriologic cultures of urine were performed. Unstained wet-mounted preparations and air-dried, modified Wright-stained urine sediment preparations were examined by light microscopy for the presence of bacteria. RESULTS: Compared with results of quantitative bacteriologic culture, routine unstained preparations and modified Wright-stained preparations had sensitivities of 82.4% and 93.2%, specificities of 76.4% and 99.0%, positive predictive values of 40.1% and 94.5%, negative predictive values of 95.8% and 98.7%, and test efficiencies of 77.3% and 98.0%, respectively. Compared with 74 samples that yielded growth on bacteriologic culture, the routine unstained method had concordance and misclassification rates of 39.2% and 60.8%, respectively, whereas the Wright-stained method had concordance and misclassification rates of 78.4% and 21.6%, respectively. Significant associations between each of occult blood in urine, pyuria, female sex, and lower urine specific gravity with bacteriuria detected by Wright-stained sediment examination and quantitative bacteriologic culture of urine were identified. CONCLUSIONS AND CLINICAL RELEVANCE: Examination of modified Wright-stained preparations of urine sediment appeared to be a rapid, cost effective method that significantly improved the sensitivity, specificity, positive predictive value, and test efficiency of light microscopic detection of bacteriuria, compared with that of the routine unstained method.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/15112776/