Peer-reviewed veterinary case report
Test for Babesia gibsoni infection in dogs using BgSA3 protein ELISA
By Vijaykumar, Lavanya K et al.·Published in Veterinary parasitology·2021·Veterinary College, India·View original on PubMed →
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Original publication title: Evaluation of recombinant BgSA3 protein based indirect-ELISA for sero-diagnosis and sero-surveillance of Babesia gibsoni in dogs.
- Species:
- dog
Plain-English summary
A study found that a new blood test can help diagnose Babesia gibsoni infection, a tick-borne disease that affects dogs. This test, based on a specific protein, was shown to be accurate, identifying 86.4% of infected dogs while avoiding false positives in healthy dogs. The researchers tested the new method on a group of dogs and found that about 12% had the infection. This test could be useful for vets to quickly and reliably diagnose this disease, helping to manage and control its spread among dogs.
People also search for: dog tick disease symptoms · Babesia gibsoni test for dogs · how to diagnose tick-borne illness in dogs
Abstract
Canine babesiosis, a tick-borne haemoprotozoan disease of dogs, is of significance globally due to its rapid spread. A precise confirmatory diagnosis is required to curtail the rapid spread of infection. Our study described the evaluation of recombinant BgSA3 protein based indirect ELISA for sero-diagnosis and sero-surveillance of Babesia gibsoni infection in dogs. A partial BgSA3 gene segment (1921 bp) of B. gibsoni, encoding for recombinant truncated BgSA3 (75 kDa) protein devoid of predicted signal peptide (23 aa) at N-terminus and transmembrane region (20 aa) at C-terminus, was expressed in E. coli using a pET28a(+) vector. The rBgSA3 protein purified under native conditions using Ni-NTA superflow cartridge was confirmed by SDS-PAGE and Western blotting using sera from dogs infected/uninfected with B. gibsoni, and erythrocyte lysate/ plasma from infected/uninfected dogs. The rBgSA3 protein was specific only to B. gibsoni antibodies but did not react with uninfected sera. Further, rBgSA3 protein was evaluated for sero-diagnosis/sero-surveillance using Indirect-ELISA format. There was no cross reactivity to B. vogeli, E. canis, H. canis and D. repens infected dogs serum samples. The diagnostic sensitivity and specificity of rBgSA3 based I-ELISA was found to be 86.4 and 93.1 % respectively, in comparison with cytb based PCR assay. Additionally, rBgSA3-ELISA evaluated using survey serum samples (n = 287), detected 11.85 % samples as positive. In conclusion, B. gibsoni infection, an emerging disease is prevalent in the present study area and the standardized rBgSA3 protein based indirect-ELISA was found to be a specific and sensitive test for large scale sero-diagnosis and sero-surveillance of B. gibsoni infection in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/33359970/