Peer-reviewed veterinary case report
Test for leptospiral antibodies in dog blood using recombinant Lig
By La-Ard, Anchalee et al.·Published in The Southeast Asian journal of tropical medicine and public health·2011·Kasetsart University·View original on PubMed →
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Original publication title: Evaluation of recombinant Lig antigen-based ELISA for detection of leptospiral antibodies in canine sera.
- Species:
- dog
Plain-English summary
A group of dogs was tested for leptospirosis, a serious infection caused by bacteria, using a new blood test that detects antibodies. The test showed good results, identifying 84.5% of dogs that had the infection compared to a standard test, while also correctly identifying 76.9% of healthy dogs. This new test is easy to prepare and could be used by veterinarians to screen for leptospirosis and confirm positive results from other tests. Overall, it offers a promising option for diagnosing this potentially dangerous disease in dogs.
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Abstract
Abstract. The objectives of this study were to clone the conserved region of leptospiral immunoglobulin-like protein (lig) gene and evaluate the utility of the recombinant Lig as an ELISA antigen for detection of leptospiral antibodies in canine sera. Leptospira kirschneri serovar Grippotyposa strain Moskva V was chosen to be a target for cloning the conserved region of Lig gene. This assay was evaluated with canine sera (n = 91) that were MAT-negative (< 1:100 dilution) and sera (n = 103) that were MAT-positive (> or = 1:100 dilution) using 24 serovars. The ELISA showed a relative sensitivity as compared to MAT of 84.5% whereas the specificity was 76.9%. This assay is simple and can be routinely prepared in large amounts. It was concluded that the GST.Lig recombinant protein-based ELISA could be used as a screening test for serodiagnosis of canine leptospirosis with also for confirmation of MAT-positive test results.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/21323175/