Evaluation of recombinant Taenia multiceps HSP70 antigen in a dipstick-based rapid diagnostic test for ovine coenurosis.

Abstract

BACKGROUND: , the larval stage of, causes coenurosis, a severe neurological disease in sheep associated with significant economic losses. Ante-mortem diagnosis remains difficult due to non-specific clinical signs and the limited applicability of laboratory-based methods under field conditions. Heat shock protein 70 (HSP70) has previously shown promising immunoreactivity in laboratory assays, suggesting its potential as a diagnostic antigen. This study aimed to evaluate the format-dependent performance of a laboratory-validated recombinant antigen when translated into a dipstick-based rapid diagnostic assay for ovine coenurosis. RESULTS: Despite strong immunoreactivity in Western blot analysis, rTmHSP70 failed to generate a visible test line signal in the dipstick assay under all tested conditions. In contrast, a partially purified hydatid cyst antigen produced visible signals but also caused non-specific reactions and false-positive results. These findings demonstrate that rTmHSP70, although suitable for laboratory-based assays, is not compatible with dipstick-format rapid diagnostics. CONCLUSION: The study underscores the importance of assay format compatibility, antigen purity, and epitope accessibility in the development of reliable field-applicable diagnostic tests for coenurosis. The findings demonstrate that strong immunoreactivity in laboratory-based assays does not necessarily translate into functionality in rapid diagnostic formats, highlighting critical format-dependent limitations in assay development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-026-05373-3.