Peer-reviewed veterinary case report
Blood and joint fluid tests for knee arthritis in dogs with ligament
By Malek, Sarah et al.·Published in PloS one·2020·Department of Veterinary Clinical Sciences, United States·View original on PubMed →
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Original publication title: Evaluation of serum MMP-2 and MMP-3, synovial fluid IL-8, MCP-1, and KC concentrations as biomarkers of stifle osteoarthritis associated with naturally occurring cranial cruciate ligament rupture in dogs.
- Species:
- dog
Plain-English summary
A group of dogs with knee arthritis caused by a torn cruciate ligament was studied to find out if certain proteins in their blood and joint fluid could help diagnose the condition. Researchers compared these dogs to healthy dogs and found that while some proteins in the blood didn't show significant differences, a protein called MCP-1 in the joint fluid was much higher in the dogs with arthritis. This suggests that measuring MCP-1 could be a useful way for vets to identify inflammation in dogs with knee arthritis. The dogs with the torn ligament underwent surgery to stabilize their knee, which is a common treatment for this issue.
People also search for: dog knee arthritis treatment · torn cruciate ligament in dogs · MCP-1 biomarker for dog arthritis
Abstract
The purpose of this study was to evaluate matrix metalloproteinases (MMP) -2 and MMP-3 in serum, and keratinocyte-derived chemoattractant (KC), interleukin 8 (IL-8) and monocyte chemoattractant 1 (MCP-1) in synovial fluid (SF) as stifle osteoarthritis (OA) biomarkers in dogs. Dogs with naturally occurring cranial cruciate ligament (CrCL) rupture (OA group) and healthy controls were recruited. Stifles with CrCL deficiency were surgically stabilized. Serum, SF, and synovial biopsy samples were collected from the OA group preoperatively, whereas samples were collected once from control dogs. A blinded veterinary pathologist graded synovial biopsies. Serum and SF analyses were performed using xMAP technology. General linear regression was used for statistical comparisons of serum biomarkers, and mixed linear regression for SF biomarkers and temporal concentration changes. The overall discriminative ability was quantified using area under curve (AUC). Spearman's correlation coefficient was used to assess correlations between synovial histology grades and the biomarkers. Samples from 62 dogs in the OA group and 50 controls were included. The MMP-2 and MMP-3 concentrations between the OA and control groups were not significantly different, and both with an AUC indicating a poor discriminative ability. All three SF biomarker concentrations were significantly different between the OA group and controls (P <0.05). The MCP-1 was the only biomarker showing an acceptable discriminative performance with an AUC of 0.91 (95% confidence interval: 0.83-0.98). The sum of the inflammatory infiltrate score was significantly correlated with all three SF biomarkers (P <0.01). Summed synovial stroma, and all scores combined were significantly correlated with IL-8 and MCP-1 concentrations (P <0.003), and the summed synoviocyte scores were significantly correlated with MCP-1 concentrations (P <0.001). Correlations between MCP-1 concentrations and synovial histopathologic grading and its discriminative ability suggest its potential as a synovitis biomarker in canine stifle OA associated with CrCL rupture.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/33211763/