Peer-reviewed veterinary case report
Stem cells on amniotic membrane help horse limb wounds heal faster
By Bassim Khashjoori et al.·Published in Iranian Journal of Veterinary Medicine·2019·Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., IR·View original on DOAJ →
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Original publication title: Evaluation of the Effects of Adipose Derived Mesenchymal Stem Cells Cultured on Decellularized Amniotic Membrane in Wound Healing of Distal Part of the Limbs in Horse
- Species:
- horse
Plain-English summary
A horse with a serious wound on its leg was treated using a special method involving stem cells from fat tissue and a decellularized amniotic membrane. The treatment aimed to speed up healing and improve the quality of the recovery. Results showed that wounds treated with the combination of the amniotic membrane and stem cells healed significantly better than those treated with just the membrane, a common ointment, or no treatment at all. The horses that received this advanced treatment showed faster healing and better tissue regeneration.
People also search for: horse leg wound treatment · stem cells for horse wounds · equine wound healing methods
Abstract
Background: Equine wounds, particularly those involving the distal portion of the limbs, often undergo prolonged complex healing and may enter a non-healing state with obvious financial and welfare implications. Poor blood supply and lower levels of oxygen to the lower limbs lead to imbalance of growth factors related to wound healing. Decellular amniotic membrane has been widely used in soft tissue engineering especially skin regeneration due to its biological and mechanical properties. It is used as a scaffold for treatment of partial full thickness wound dressing. OBJECTIVES: Evaluation of the effects adipose derived mesenchymal cells (ADMSCs) cultured on decellularized amniotic membrane (DAM) in wound healing of distal part of equine limb. METHODS: Stem cells were isolated from adipose tissue in the base of the tail and then cultured and seeded on bovine decellular amniotic membrane. Full thickness excision wounds (2x3 cm) were created on the mid-lateral of all metatarsus of each horse aseptically. The wounds were classified into four groups: the first one covered by DAM seeded by ADMSCs, the second one dressed with only DAM, the third one treated with1% silver sulfadiazine, and the last one as control with no treatment. Digital photographs of wounds were obtained at each bandage change while biopsy samples were obtained for histopathology at days 5, 15 and 30 after treatment. RESULTS:Wounds treated with DAM/ADMSCs significantly demonstrated promotion of wound healing in geometric analysis of total wound area, epithelialization and wound contraction compared with the other groups (P<0.05). Microscopically, re-epithelization of wounds covered by DAM/ADMSCs and DAM at day 5 was significantly more than Control group (P<0.05). Fibroplasia in wounds treated with DAM/ADMSCs and DAM was higher than the group that received sulfadiazine (P<0.05). CONCLUSIONS: These results indicate positive effects of using DAM to improve the quality and speed of wound healing. Use of DAM as a scaffold for ADMSCs shows better results.
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Search related cases →Original publication on DOAJ: https://doi.org/10.22059/ijvm.2019.244278.1004854