Peer-reviewed veterinary case report
Accuracy of parvovirus and coronavirus tests in puppies with diarrhea
By Dik, Irmak et al.·Published in Eurasian Journal of Veterinary Sciences·2023·View original on Crossref →
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Original publication title: Evaluation of the Performance of Diagnostic Methods of Canine Parvovirus-2 and Canine Enteric Coronavirus Infections under Different Storage Conditions and Determination of Molecular Characterization
- Species:
- dog
Plain-English summary
A group of unvaccinated puppies aged 0-12 months with diarrhea were tested for two viral infections: canine parvovirus (CPV) and canine enteric coronavirus (CCoV). Researchers found that while both rapid tests and PCR tests could detect CPV effectively in fresh stool samples, the rapid test was less reliable for CCoV, especially after the samples were frozen and thawed. The study highlighted that using fresh samples and molecular testing methods is crucial for accurately diagnosing these infections.
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Abstract
Aim: This research was carried out to detect CPV and CCoV infections in dogs in comparison with rapid kit and PCR and to determine the molecular characterization of these infections in Konya region. Besides, it was aimed to determine the sensitivity and specificity rates of the diagnostic tests after fresh or freeze-thawed stool for infection diagnosis. Materials and Methods: Faecal samples were collected from 50 unvaccinated, 0-12 months old dogs with diarrhoea symptoms at the shelter. The samples were analysed for CPV and CCoV by rapid test and PCR test. After freezethawing, the samples were checked again with the same tests. Results: CPV was positively diagnosed by rapid test and PCR in 2 and 29 fresh stool samples, respectively, and CCoV in 14 and 28 samples. CPV positive samples did not change while CCoV was diagnosed as positive in 10 samples and 28 samples by rapid test and PCR, respectively, after the freeze-thaw procedure. Although there were no differences in the diagnosis of CPV, the sensitivity of the rapid test in the diagnosis of CCoV decreased after the freezethaw procedure. In addition, only CPV-2b type was detected in CPV positive samples and both GI and GII subtypes were detected in CCoV positive samples as molecular. In conclusion, it was observed that rapid tests are not sensitive for accurate diagnosis of CPV and CCoV infections. Conclusion: The importance of choosing molecular diagnostic methods and using fresh samples for accurate diagnosis of virological infections can be emphasized.
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Search related cases →Original publication on Crossref: https://doi.org/10.15312/eurasianjvetsci.2023.412