Peer-reviewed veterinary case report
Evaluation of the recombinant 10-kilodalton immunodominant region of the BP26 protein of Brucella abortus for specific diagnosis of bovine brucellosis.
- Journal:
- Clinical and vaccine immunology : CVI
- Year:
- 2011
- Authors:
- Tiwari, Arvind Kumar et al.
- Affiliation:
- Division of High Containment Facility · India
- Species:
- cat
Plain-English summary
Brucellosis is a disease that affects both animals and humans, and it is caused by a bacteria called Brucella abortus in cows. Current tests for this disease can sometimes give false-positive results, meaning they might incorrectly suggest that a cow is infected when it isn't. Researchers have been working on a new test using a specific protein from the bacteria, which seems to be more accurate. In their study, they tested this new method on 408 cow blood samples and found that it correctly identified infected cows without mistakenly labeling vaccinated cows as infected. The results suggest that this new test could be a reliable way to diagnose bovine brucellosis and distinguish between vaccinated and infected animals.
Abstract
Brucellosis is a disease with worldwide distribution affecting animals and human beings. Brucella abortus is the causative agent of bovine brucellosis. The cross-reactions of currently available diagnostic procedures for B. abortus infection result in false-positive reactions, which make the procedures unreliable. These tests are also unable to differentiate Brucella-infected and -vaccinated animals. The present work is focused on the use of a nonlipopolysaccharide (LPS) diagnostic antigen, a recombinant 10-kDa (r10-kDa) protein of B. abortus, for specific diagnosis of brucellosis. The purified recombinant protein was used as a diagnostic antigen in plate enzyme-linked immunosorbent assay (p-ELISA) format to screen 408 bovine serum samples (70 presumptively negative, 308 random, and 30 vaccinated), and the results were compared with those of the Rose Bengal plate agglutination test (RBPT) and the standard tube agglutination test (STAT). Statistical analysis in presumptive negative samples revealed 100 and 98.41% specificity of p-ELISA with RBPT and STAT, and an agreement of 91.43% with the tests using Cohen's kappa statistics. In random samples, the agreement of p-ELISA was 77.92% and 80.52% with RBPT and STAT, respectively. p-ELISA investigation of vaccinated samples reported no false-positive results, whereas RBPT and STAT reported 30% and 96.6% false-positive results, respectively. The data suggest that p-ELISA with r10-kDa protein may be a useful method for diagnosis of bovine brucellosis. Furthermore, p-ELISA may also be used as a tool for differentiating Brucella-vaccinated and naturally infected animals.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21852548/