Peer-reviewed veterinary case report
Evaluation of two magnetic-bead-based viral nucleic acid purification kits and three real-time reverse transcription-PCR reagent systems in two TaqMan assays for equine arteritis virus detection in semen.
- Journal:
- Journal of clinical microbiology
- Year:
- 2011
- Authors:
- Miszczak, Fabien et al.
- Affiliation:
- Animal Health Department · France
- Species:
- horse
Plain-English summary
This study looked at different methods for detecting equine arteritis virus in horse semen. Researchers compared two kits for extracting viral genetic material and three testing systems to see which was most effective. They found that one specific testing method, called the T1 assay, was just as good or even better than traditional virus isolation techniques for finding the virus. This means that using the T1 assay could be a reliable option for testing for equine arteritis virus in horses.
Abstract
This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21832018/