Exercise Stimulates, andGenes Expression Despite High-fat Diet: Tissue-specific Responses.

Abstract

BACKGROUND/AIM: Adipose tissue is pivotal in regulating metabolism, functioning not only as an energy store but also as an endocrine organe. Our investigation focused on the gene expression levels ofandin C57BL/6J mice subjected to a high-fat diet and exercise regimen. The C57BL/6J mouse model was chosen due to its well-documented genetic background to diet-induced obesity for studying obesity. MATERIALS AND METHODS: The study consisted of three groups: control (C), high-fat diet (HFD), and exercise-high fat diet (E-HFD) groups. While HFD and E-HFD mice received a 60% fat diet, controls received a 10% fat diet. Real-time polymerase chain reaction system was applied to evaluate gene expression levels in hepatic, adipose, and heart tissues. Pro-inflammatory cytokine levels in the serum were quantified using ELISA kits according to the protocols. RESULTS: In contrast to the control and HFD groups, the E-HFD group exhibited notably elevated gene expressions ofand(<0.05). In the hepatic tissue of E-HFD group, gene expression ofandwere significantly upregulated (<0.05). However, there was a significant reduction inandgene expression in the hepatic tissues of the HFD group (<0.05). CONCLUSION: Exercise and high-fat diet intervention significantly alter expression levels of mitophagy-related genes, andand suggest that the examined genes may serve as potential molecular candidates for further research on obesity-related mitochondrial dysfunction and exercise-mediated metabolic improvement.