Experimental models and methods for infected wound modeling with Staphylococcus aureus.

Abstract

Acute or chronic non-healing wounds place nowadays a massive financial burden on healthcare systems. Although we have gained much knowledge about the fundamental molecular and cellular mechanisms that promotes healthy, acute wound healing from various animal models, further preclinical research is necessary to explore the pathology and role of infection in the development and persistence of both acute and chronic non-healing wounds. In this study we present a comprehensive protocol for the generation of an infected acute or chronic non-healing wound on a mouse model that could serve as a useful tool for uncovering pathological mechanisms and potential therapeutic targets for eventual translation to the clinic.