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Peer-reviewed veterinary case report

Exploring the Role of ADAMTS4 Expression in Atherosclerosis.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2026
Authors:
Aswani, S S et al.
Affiliation:
Department of Biochemistry · India

Abstract

ADAMTS4 is a secretory metalloproteinase involved in the pathogenesis of atherosclerosis. Both in vitro and in vivo model systems are used to study the regulation of ADAMTS4 in atherosclerosis. Here we describe three model systems: in vitro model system of macrophages and foam cells and in vivo model of high-fat-diet-induced atherosclerosis. We can use qPCR for studying the mRNA expression of ADAMTS4, while ELISA and Western blot can be employed to assess its protein expression.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/41917353/