Peer-reviewed veterinary case report
Expression and immunogenic comparison of VP2 and VP3 from marine birnavirus.
- Journal:
- Journal of fish diseases
- Year:
- 2008
- Authors:
- Xu, H-J et al.
- Affiliation:
- College of Agriculture & Biotechnology · China
- Species:
- rabbit
Abstract
The coding regions for the major epitopes of structural protein VP2 (vp2e) and structural protein VP3 were amplified from marine birnavirus (MABV) cDNA and efficiently expressed as glutathione S-transferase (GST) fusion proteins in Escherichia coli. Polyclonal antibodies against VP2e and VP3 were raised in rabbits and fish using the purified proteins of GST/VP2e and GST/VP3. The rabbit anti-serum against VP3 was more sensitive than the rabbit anti-VP2e serum in detecting virus in MABV-infected fish, while fish anti-VP2e serum showed a stronger neutralization response than fish anti-VP3 serum.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18353021/