Expression kinetics of ISG15 and viral major capsid protein (VP2) in Atlantic cod (Gadus morhua L.) fry following infection with infectious pancreatic necrosis virus (IPNV).

Abstract

Atlantic cod fry (1g) were infected by intraperitoneal injection with IPNV and samples of liver were taken every second day from four fish up to day 21. Samples were analysed for levels of viral transcripts by real time RT-PCR and the induction of expression of interferon stimulated gene 15 (ISG15) transcripts were estimated by conventional RT-PCR relative to beta-actin. Mortality of over 40% occurred in infected groups between day 6 and 12 after infection. Levels of viral transcripts were low on day 1, rose on day 3, peaked on day 5 remaining high till day 13, and thereafter declined to low levels by day 21. The highest levels of viral transcripts, therefore, coincided with the onset and duration of mortality, but low levels persisted in surviving fish. ISG15 transcripts in control fish were detectable at low levels. Following infection with IPNV there was a marked increase in transcripts on day 3 and this level persisted up to day 21. This is the first report that IPNV induces the expression of the ISG15 gene in Atlantic cod.