Peer-reviewed veterinary case report
E-cadherin protein levels in duodenal tissue of dogs
By Ohta, Hiroshi et al.·Published in American journal of veterinary research·2014·Department of Veterinary Clinical Sciences, Japan·View original on PubMed →
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Original publication title: Expression of apical junction complex proteins in duodenal mucosa of dogs with inflammatory bowel disease.
- Species:
- dog
Plain-English summary
A group of dogs with inflammatory bowel disease (IBD) had biopsies taken from their intestines to study the proteins involved in gut health. The researchers found that while most proteins were similar between healthy dogs and those with IBD, a protein called E-cadherin was significantly lower in the dogs with IBD. This suggests that lower levels of E-cadherin might contribute to the problems seen in dogs with IBD. Understanding these differences could help veterinarians develop better treatments for dogs suffering from this condition.
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Abstract
OBJECTIVE: To determine the expression of tight junction and adherens junction proteins in duodenal mucosa samples of dogs with inflammatory bowel disease (IBD). ANIMALS: 12 dogs with IBD and 6 healthy control Beagles. PROCEDURES: Duodenal mucosa biopsy samples were endoscopically obtained from dogs with IBD and healthy control Beagles. The expression of claudin-1, -2, -3, -4, -5, -7, and -8; E-cadherin; and β-catenin in the duodenal mucosa samples was determined by means of immunoblotting. The subcellular localization of E-cadherin in the duodenal mucosa samples was determined with immunofluorescence microscopy. RESULTS: The expression of each claudin and β-catenin was not significantly different between control dogs and dogs with IBD. However, expression of E-cadherin was significantly lower in duodenal mucosa samples of dogs with IBD than it was in samples obtained from healthy control dogs. Results of immunofluorescence microscopy indicated decreased intensity of E-cadherin labeling in the tips of villi in duodenal mucosa samples obtained from 6 dogs with IBD, compared with staining intensity for other dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Results of this study indicated expression of claudin-1, -2, -3, -4, -5, -7, and -8 and β-catenin was not significantly different between duodenal mucosa samples obtained from control dogs and those obtained from dogs with IBD. However, E-cadherin expression was significantly lower in the villus epithelium in duodenal mucosa samples obtained from dogs with IBD versus samples obtained from control dogs, which suggested that decreased expression of that protein has a role in the pathogenesis of IBD in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25061706/