Peer-reviewed veterinary case report
Immune gene changes in dogs with stifle arthritis and ligament rupture
By Muir, P et al.·Published in Veterinary immunology and immunopathology·2007·School of Veterinary Medicine, United States·View original on PubMed →
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Original publication title: Expression of immune response genes in the stifle joint of dogs with oligoarthritis and degenerative cranial cruciate ligament rupture.
- Species:
- dog
Plain-English summary
A group of dogs with oligoarthritis (a type of inflammatory arthritis) and torn cranial cruciate ligaments (CCL) showed increased levels of certain immune response genes in their joint fluid compared to healthy dogs. This suggests that their immune systems might be reacting in a way that contributes to ongoing joint inflammation and damage. The study found that specific genes related to inflammation and tissue breakdown were more active in these dogs, indicating a potential link between immune response and joint problems. Understanding this could help veterinarians develop better treatments for dogs suffering from similar conditions.
People also search for: dog oligoarthritis treatment · torn CCL in dogs · dog joint inflammation symptoms
Abstract
Dysregulation of immune responses within joints plays an important role in development of inflammatory arthritis. We determined expression of a panel of immune response and matrix turnover genes in synovial fluid collected from a group of dogs with stifle oligoarthritis and associated degenerative cranial cruciate ligament (CCL) rupture (n=27). We also studied synovial fluid gene expression in dogs affected with other forms of degenerative arthritis (n=9) and in the stifle joint of healthy dogs with intact CCL (n=14). After collection, synovial cells were pelleted and RNA was isolated. Relative expression of cathepsin K, cathepsin S, tartrate-resistant acid phosphatase (TRAP), matrix metalloproteinase-9 (MMP-9), invariant chain (li), toll-like receptor-2 (TLR-2), and TLR-9 was determined using real-time quantitative RT-PCR. Data were normalized to peripheral blood mononuclear cells (PBMC) as an internal control. Relative expression of cathepsin K, MMP-9, TRAP, and li was increased in the stifle synovial fluid of dogs with oligoarthritis, when compared with the stifles of healthy dogs (P<0.05). In contrast, relative expression of all of the genes-of-interest in synovial fluid from joints affected with other forms of arthritis was not significantly different from the stifles of healthy dogs. TRAP expression was also significantly increased in the stifle joints of dogs with oligoarthritis, when compared to joint expression of TRAP in dogs with other forms of degenerative arthritis (P<0.05). In the dogs with stifle oligoarthritis, expression of both matrix turnover and immune response genes was increased in stifle synovial fluid, when compared with the internal PBMC control, whereas in healthy dogs and dogs with other forms of arthritis, only expression of matrix turnover genes was increased in synovial fluid, when compared with the internal PBMC control (P<0.05). Taken together, these findings suggest that antigen-specific immune responses within the stifle joint may be involved in the pathogenesis of persistent synovitis and associated joint degradation in dogs with oligoarthritis and degenerative CCL rupture.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/17629954/