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Peer-reviewed veterinary case report

Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and evaluation of its protective effects.

Journal:
The Journal of parasitology
Year:
2005
Authors:
Shirafuji, Hiroaki et al.
Affiliation:
Obihiro University of Agriculture and Veterinary Medicine · Japan
Species:
rodent

Abstract

In this study, P23 of Cryptosporidium parvum sporozoites, an immunodominant surface protein, was stably expressed in Toxoplasma gondii (Tg/P23) and its protective effects were evaluated in a mouse model. The molecular weight and antigenic property of P23 expressed by Tg/P23 were similar to those of the native P23. Mice immunized with lysed Tg/P23 tachyzoites produced specific neutralizing antibodies against C. parvum. These findings indicate that the T. gondii vector may provide a new tool for the production of a recombinant vaccine against cryptosporidiosis in animals.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/15986633/