Peer-reviewed veterinary case report
Protein differences in joint fluid and tissue of dogs with knee
By Garner, Bridget C et al.·Published in American journal of veterinary research·2013·University of Missouri, United States·View original on PubMed →
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Original publication title: Expression of proteins in serum, synovial fluid, synovial membrane, and articular cartilage samples obtained from dogs with stifle joint osteoarthritis secondary to cranial cruciate ligament disease and dogs without stifle joint arthritis.
- Species:
- dog
Plain-English summary
A group of dogs with stifle joint osteoarthritis, often caused by cranial cruciate ligament (CCL) disease, had their joint fluid and blood samples analyzed to find differences in protein levels compared to healthy dogs. The study found that certain proteins related to inflammation and metabolism were more present in the joint fluid of dogs with arthritis. However, there were no significant differences in the blood samples. These findings suggest that specific proteins could be involved in the development of joint arthritis in dogs.
People also search for: dog arthritis symptoms · stifle joint osteoarthritis treatment · cranial cruciate ligament disease in dogs
Abstract
OBJECTIVE: To identify proteins with differential expression between healthy dogs and dogs with stifle joint osteoarthritis secondary to cranial cruciate ligament (CCL) disease. SAMPLE: Serum and synovial fluid samples obtained from dogs with stifle joint osteoarthritis before (n = 10) and after (8) surgery and control dogs without osteoarthritis (9) and archived synovial membrane and articular cartilage samples obtained from dogs with stifle joint osteoarthritis (5) and dogs without arthritis (5). PROCEDURES: Serum and synovial fluid samples were analyzed via liquid chromatography-tandem mass spectrometry; results were compared against a nonredundant protein database. Expression of complement component 3 in archived tissue samples was determined via immunohistochemical methods. RESULTS: No proteins had significantly different expression between serum samples of control dogs versus those of dogs with stifle joint osteoarthritis. Eleven proteins (complement component 3 precursor, complement factor I precursor, apolipoprotein B-100 precursor, serum paraoxonase and arylesterase 1, zinc-alpha-2-glycoprotein precursor, serum amyloid A, transthyretin precursor, retinol-binding protein 4 precursor, alpha-2-macroglobulin precursor, angiotensinogen precursor, and fibronectin 1 isoform 1 preproprotein) had significantly different expression (> 2.0-fold) between synovial fluid samples obtained before surgery from dogs with stifle joint osteoarthritis versus those obtained from control dogs. Complement component 3 was strongly expressed in all (5/5) synovial membrane samples of dogs with stifle joint osteoarthritis and weakly expressed in 3 of 5 synovial membrane samples of dogs without stifle joint arthritis. CONCLUSIONS AND CLINICAL RELEVANCE: Findings suggested that the complement system and proteins involved in lipid and cholesterol metabolism may have a role in stifle joint osteoarthritis, CCL disease, or both.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23438113/