Peer-reviewed veterinary case report
Fecal changes in dogs with diarrhea after transplant or antibiotics
By Chaitman, Jennifer et al.·Published in Frontiers in veterinary science·2020·Veterinary Internal Medicine and Allergy Specialists, United States·View original on PubMed →
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Original publication title: Fecal Microbial and Metabolic Profiles in Dogs With Acute Diarrhea Receiving Either Fecal Microbiota Transplantation or Oral Metronidazole.
- Species:
- dog
Plain-English summary
A group of dogs with acute diarrhea were treated with either a fecal microbiota transplant (FMT) or oral metronidazole for a week. By the end of the treatment, both groups showed improvement in their stool consistency, but those receiving the FMT had better results compared to the metronidazole group after 28 days. The FMT group also showed a return to healthier gut bacteria and metabolism, while the metronidazole group still had signs of gut imbalance. This suggests that fecal microbiota transplantation may be a more effective treatment for dogs with acute diarrhea than metronidazole.
People also search for: dog diarrhea treatment · fecal microbiota transplant for dogs · metronidazole for dog diarrhea
Abstract
The aim was to characterize differences in fecal consistency, and fecal microbiota and metabolome profiles in dogs with acute diarrhea (AD) treated with either fecal microbiota transplantation as enema (FMT;= 11) or oral metronidazole (MET;= 7) for 7 days. On days 0, 7, and 28 fecal samples were obtained. Fecal samples from healthy dogs (HC;= 14) were used for comparison. Samples were analyzed by the previously validated qPCR based canine Dysbiosis Index (DI; increased values indicate microbiota dysbiosis) and 16S rRNA gene sequencing. The fecal metabolome was analyzed using a previously validated targeted canine assay for fecal unconjugated bile acids, and untargeted metabolomics. Fecal consistency improved significantly in dogs treated with FMT and MET by day 7 and day 28 (< 0.01) compared to day 0. However, on day 28 fecal consistency was significantly better in FMT compared to MET (= 0.040). At day 0, dogs with AD had an altered microbiota indicated by significantly increased DI, decreased alpha-diversity, and altered beta-diversity. In the FMT group, the DI decreased over time, while MET led to a significant increase in the dysbiosis index at day 7 and 28 compared to FMT. Sequencing data revealed that in FMT microbial diversity and beta-diversity was similar to HC at day 28, while in MET these parameters were still significantly different from HC. In dogs treated with FMT, a decrease in cholic acid and the percentage of primary bile acids was observed, whereas treatment with metronidazole led to an increase in cholic acid at day 7 and an increase in percentage of primary bile acids over time. Based on untargeted metabolomics, dogs with AD had an altered fecal metabolome compared to HC. Dogs treated with FMT clustered closer to HC at day 28, while dogs treated with MET did not. In this pilot study, dogs with AD had significant differences in fecal microbiota and metabolome profiles. Dogs treated with MET still had altered microbial and metabolic profiles at day 28 compared to dogs treated with FMT or healthy dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/32363202/