First Molecular Evidence of Anaplasma phagocytophilum in Rodent Populations of Nanchang, China.

Abstract

In this study, systematic surveillance of rodent populations in Nanchang of China and determination of Anaplasma phagocytophilum infection in rodents were performed. Between 2011 and 2015, 110,084 rodent snap traps were set in 4 counties and in the city center of Nanchang, China. Finally, 942 rodents were captured, with a relative density of 0.86%. The densities varied considerably by geographical area with Anyi being the most rodent-infested County. Frequently captured rodents were sewer rats (Rattus norvegicus), house mice (Mus musculus), and Rattus flavipectus. The Anaplasma genera were investigated by PCR in 19 live rodents trapped by welded cages in Anyi, 6 rodents were assessed as positive based on amplification of 16S rRNA. Sequence analysis revealed 3 variants of A. phagocytophilum in Nanchang. PCR analysis of the gltA (citrate synthase) gene found 1 sample that was positive for A. phagocytophilum infection. The sequence of A. phagocytophilum gltA gene formed a clade with and showed 99% identity to A. phagocytophilum that has been previously described in rodents from South-Eastern China. Taken together, our research indicated that commensal rodents are potential hosts for A. phagocytophilum and controlling the rodent population may facilitate subsequent prevention of human granulocytic anaplasmosis in Nanchang, China, in the future.