Fucosterol alleviated intestinal inflammation of spotted seabass (Lateolabrax maculatus) involving in the improvement of endoplasmic reticulum stress-mediated mitochondrial dysfunction.

Abstract

A 56-day feeding trial was conducted to examine the impact of dietary fucosterol (FSL) supplementation on intestinal inflammation in spotted seabass. 540 fish (initial average weight: 9.33 ± 0.06 g) were fed six diets with varying FSL levels (0.0%, 0.5%, 1.0%, 1.5%, 2.0% and 2.5%) and subsequently challenged with Aeromonas hydrophila for ten days. The findings demonstrated that FSL supplementation enhanced growth performance, reduced enteritis morbidity and intestinal histopathological lesions. Moreover, FSL supplementation in diet decreased the mRNA expressions of most pro-inflammatory cytokines, while increasing the mRNA expressions of anti-inflammatory cytokines. The reduction of intestinal inflammation was achieved by mitigating endoplasmic reticulum (ER) stress through the inhibition of ire1α/xbp1s, perk/eif2α/chop and atf6 pathways. ER stress alleviation may be associated with the regulation of mitochondrial quality control, including the promotion of mitochondrial biogenesis (activating AMPK/Sirt1//PGC-1α pathway), fusion (up-regulating mfn1 and opa1 expressions except mfn1b) and autophagy (up-regulating LC3B expression and down-regulating p62 expression related to activate pink1/parkin pathway) as well as the inhibition of mitochondrial fission (down-regulating drp1 and fis1 expressions). Finally, we determined the optimal levels of FSL for percent weight gain (PWG) and enteritis morbidity in spotted seabass as 1.42% and 1.74%, respectively, and using them as the indicators for FSL supplementation. Overall, FSL supplementation may be an effective strategy to improve intestinal inflammation in fish.