Functionalization of Polyvinylpyrrolidone Films by Grafting Maleic Acid from PVP Gels for Loading Studies of Naringin and Silver Nanoparticles as Potential Wound Dressings.

Abstract

Wound healing is a complex process involving stages such as hemostasis, inflammation, proliferation, and remodeling. In this context, polymers are useful materials for wound treatment. This research used the Casting method to prepare films from 2% polyvinylpyrrolidone (PVP) gels. Subsequently, PVP films were grafted with maleic acid (MA) (PVP-g-PAM) to load naringin (NA) and silver nanoparticles (AgNPs) in order to obtain a material with pH responsiveness and antibacterial properties. The modified PVP-g-PAM films were prepared using gamma-ray irradiation through a pre-irradiation oxidative method at a dose rate of 13.7 kGy h^-1, doses ranging from 10 to 25 kGy, and reaction times from 50 to 80 min in a bath of water, all samples at 50 °C, and a fixed monomer concentration of 15% (<i>w</i>/<i>v</i>) MA in THF. The conditions that yielded the highest percentage of grafting were 20 kGy and 60 min. NA was loaded at a fixed concentration of 5%. Data release showed that the films follow the Korsmeyer-Peppas kinetic model. Synthesis of AgNPs was performed by γ-ray irradiation-reduction (10 and 30 kGy), using PVP as a stabilizer. AgNPs showed in vitro effectiveness against <i>E. coli</i> and <i>S. aureus</i>. Films were characterized by FTIR-ATR, TGA, DSC, mechanical properties, swelling index, and contact angle. Further studies must be implemented; however, the results up now suggest that PVP-g-PAM loaded with NA and AgNPs can be useful as a potential wound dressing.