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Peer-reviewed veterinary case report

Generation and Characterization of Mouse Models for Skeletal Disease.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2021
Authors:
Foxa, Gabrielle E et al.
Affiliation:
Van Andel Institute · United States
Species:
rodent

Abstract

Our laboratories have used genetically engineered mouse models (GEMMs) to assess genetic contributions to skeletal diseases such as osteoporosis and osteoarthritis. Studies on the genetic contributions to OA are often done by assessing how GEMMs respond to surgical methods that induce symptoms modeling OA. Here, we will describe protocols outlining the induction of experimental OA in mice as well as detailed descriptions of methods for analyzing skeletal phenotypes using micro-computerized tomography and skeletal histomorphometry.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/32979204/