Peer-reviewed veterinary case report
Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing.
- Journal:
- Journal of virological methods
- Year:
- 2024
- Authors:
- Brinkmann, Annika et al.
- Affiliation:
- Robert Koch Institute · Germany
Abstract
We present an amplicon-based assay for MinION Nanopore sequencing of mpox virus (MPXV) genomes from clinical specimens, obtaining high-quality results with an average genome coverage of 99% for Ct values of up to 25, and a genome coverage of 97.1% for Ct values from 25 to 30 which are challenging to sequence. This assay is easy to implement in PCR-based workflows and provides accurate genomic data within a short time.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/38246565/