Genome-wide characterization, phylogenetic and expression analysis ofgene family in Golden pompano.

Abstract

Galectins (Gals) are a type of S-type lectin that are widespread and evolutionarily conserved among metazoans, and can act as pattern recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). In this study,() were identified in the Golden pompano (), and their conserved domains, motifs, and collinearity relationships were analyzed. The expression ofwas regulated following infection toand, indicating thatparticipate in immune responses against microbial pathogens. Further analysis was conducted on one important member, Galectin-3, subcellular localization showing that ToGal-3like protein is expressed both in the nucleus and cytoplasm. Recombinant protein obtained through prokaryotic expression showed that rToGal-3like can agglutinate red blood cells of rabbit, carp and golden pompano and also agglutinate and kill, and. This study lays the foundation for further research on the immune roles ofin teleosts.