Peer-reviewed veterinary case report
Multidrug-resistant Klebsiella infection in dog with chronic ear
By Fábio P. Sellera et al.·Published in Journal of Global Antimicrobial Resistance·2020·Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil, GB·View original on DOAJ →
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Original publication title: Genomic analysis of multidrug-resistant CTX-M-15-positive Klebsiella pneumoniae belonging to the highly successful ST15 clone isolated from a dog with chronic otitis
- Species:
- dog
Plain-English summary
A dog with chronic ear infections (otitis) was found to have a strain of Klebsiella pneumoniae that is resistant to multiple antibiotics. This strain, known as CTX-M-15-positive K. pneumoniae, is part of a successful group of bacteria that can spread easily and cause infections in both pets and humans. The genetic analysis revealed that this strain carries several resistance genes, making it difficult to treat. This case highlights the importance of monitoring antibiotic-resistant bacteria in veterinary settings to ensure better treatment options for pets.
People also search for: dog ear infection treatment · antibiotic-resistant bacteria in dogs · chronic otitis in dogs
Abstract
Background: Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae have been increasingly reported worldwide as a frequent cause of human and animal infections. K. pneumoniae belonging to the K24 capsular serotype and sequence type (ST) ST15 has been considered a global successful clone responsible for the spread of the blaCTX-M-15 gene. Objective: To report the draft genome sequence of a multidrug-resistant CTX-M-15-positive K. pneumoniae K24-ST15 strain (L3KP1), which was isolated from a dog with chronic otitis. Methods: Genomic DNA was extracted and sequenced using Illumina NextSeq platform. De novo assembly was performed by SPAdes and in silico prediction accomplished by curated bioinformatics tools. Results: The genome size was calculated at 5 642 348 bp, with a GC content of 57.11%, and comprising 5601 total genes, 52 tRNAs, 8 rRNAs, 9 ncRNAs and 105 pseudogenes. The K. pneumoniae L3KP1 strain belonged to ST15 and carried the yersiniabactin biosynthetic gene cluster [ybt 10 (YbST28) in the integrative conjugative element ICEKp4], and the KL24 locus encoding capsular serotype K24. Besides the blaCTX-M-15 ESBL gene, other clinically important resistance genes to β-lactams, aminoglycosides, fosfomycin, macrolides, phenicol, quinolones, sulfonamides, tetracyclines and trimethoprim were detected. Additionally, heavy metals and disinfectant resistance genes were also identified. Conclusion: This draft genome might be useful for comparative genomic analyses of the international clone of K. pneumoniae K24-ST15-CTX-M-15. In addition, information presented in this study also shed light on the urgent need to monitor ESBL-producing K. pneumoniae in veterinary hospitals.
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Search related cases →Original publication on DOAJ: https://doi.org/10.1016/j.jgar.2020.06.017