Genomic Characterization of Multidrug-Resistant Myroides Odoratimimus from Pneumonic Piglets: A One Health Threat.

Abstract

Myroides odoratimimus is an emerging opportunistic pathogen increasingly implicated in infections across human and animal populations. We previously reported the first outbreak of pneumonia in post-weaning piglets associated with M. odoratimimus, marking a significant shift in its recognized host range. The affected swine herd exhibited co-infection with Porcine Circovirus types 2 and 3 (PCV2 and PCV3), likely contributing to proliferative dermatitis and nephropathy syndrome (PDNS), reproductive abnormalities and immunosuppression. This study presents the genomic characterization of a multidrug-resistant strain (M. odoratimimus pgdne) isolated during the outbreak. Whole-genome sequencing revealed multiple antimicrobial resistance determinants-including the chromosomally encoded blaMUS-1 metallo-β-lactamase-and several virulence-associated factors. Genotype-phenotype correlation demonstrated strong concordance. Phylogenetic analysis (distance method, 1,000 bootstrap replicates) showed close relatedness with human isolates, suggesting zoonotic potential. These findings underscore the pathogenic capacity of M. odoratimimus in immunocompromised animal hosts and highlight its relevance to public health and thereby reinforces the need for integrated One Health-based surveillance and control strategies targeting emerging multidrug-resistant pathogens.