Genomic epidemiology of the opportunistic pathogenfrom companion dogs.

Abstract

(formerlysubsp.) is a common commensal and opportunistic pathogen of companion dogs. It carries a range of antimicrobial resistance genes and is an occasional zoonotic pathogen.Despite the potential insight offered by genome sequencing into the biology of, few genomes are currently available for study.To sequence and analysegenomes to improve understanding of this organism's molecular epidemiology, antimicrobial resistance and bacterium-host interactions.Twenty-five genomes of clinical isolates collected at a veterinary referral hospital in Scotland, UK, were sequenced with Illumina technology. These genomes were analysed by a series of bioinformatics tools along with 16 previously sequenced genomes.Phylogenetic comparison of the 41 genomes shows that the currentphylogeny is dominated by clades of closely related isolates, at least one of which has spread internationally. Ten of the 11 methicillin-resistantgenomes in this collection of 41 encoded thepromoter and gene mutations that are predicted to render the isolates susceptible to penicillins in the presence of clavulanic acid, a feature only described to date in methicillin-resistant. Seven such isolates were from the current study and, in line with the genome-based prediction, all were susceptible to amoxicillin/clavulanic acidshared very few highly conserved virulence-associated genes with, another common commensal and opportunistic canine pathogen.The availability of a further 25 genome sequences from clinicalisolates will aid in better understanding the epidemiology, bacterial-host interactions and antimicrobial resistance of this opportunistic pathogen.