Peer-reviewed veterinary case report
Genomic hybrid capture assay to detect: an application to diagnose neuroborreliosis in horses.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2022
- Authors:
- Divers, Thomas J et al.
- Affiliation:
- College of Veterinary Medicine · United States
- Species:
- horse
Abstract
Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing forin CSF and the low specificity of serum:CSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for theflagellin gene was negative. However, we enrichedDNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identifiedin the CSF of the horse, confirming a diagnosis of neuroborreliosis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35864735/