Peer-reviewed veterinary case report
Genotype-specific Taqman® assays for the detection and rapid characterisation of European strains of viral haemorrhagic septicaemia virus.
- Journal:
- Journal of virological methods
- Year:
- 2013
- Authors:
- Bland, Fiona et al.
- Affiliation:
- Marine Scotland Science · United Kingdom
Plain-English summary
This study focused on a virus called viral haemorrhagic septicaemia virus (VHSV), which causes serious illness and death in fish, particularly in European rainbow trout. Researchers developed a new testing method that can quickly identify different types of this virus based on its genetic makeup. They tested this method on 66 virus samples and found that it was very accurate and sensitive, allowing for quick identification of specific virus types and even mixed types in samples. This new testing tool will be useful for monitoring the spread of the virus and helping to manage fish health in aquaculture settings. Overall, the treatment method they developed worked well for its intended purpose.
Abstract
Viral haemorrhagic septicaemia virus (VHSV) is the agent of a disease that causes mortality events in marine and freshwater fish. It is one of the most important pathogens in European rainbow trout (Oncorhynchus mykiss) aquaculture. Four major genotypes of the virus are recognised reflecting different geographic and host ranges. Genotyping of VHS isolates is important for disease management enabling monitoring of disease spread into new geographical regions or susceptible species. This study sought to develop molecular tools for rapid and efficient classification of European VHSV genotypes. Specificity of genotype-specific real-time reverse transcription polymerase chain reaction (RT-qPCR) assays targeting the viral nucleoprotein (N) gene was tested using 66 viral isolates. All designed Taqman(®) RT-qPCR assays were genotype specific, displayed a high sensitivity and together constituted a diagnostic method for the rapid discrimination of European VHSV genotypes. Practical diagnostic applications of such assays demonstrated in this study include: (1) rapid genotype determination of isolates; and (2) identification of mixed-genotype isolates originating from pooled samples in areas where genotype distribution is known to overlap. However, the most important application will be supporting international VHSV surveillance programmes through the provision of a rapid specific and sensitive isolate characterisation method.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23089575/