Genotyping and study of adherence-related genes of Streptococcus uberis isolates from bovine mastitis.

Abstract

The aim of this study was to determine the presence, conservation, and distribution of 6 potential adherence genes and their relationship with diverse molecular types in 34 S. uberis isolated from bovine mastitis in Argentina. Pulsed-field gel electrophoresis (PFGE) typing with SmaI was performed. The PCR for the detection of each gene, scpA, acdA, fbp, lbp, lmb, and sua was standardized. Samples of the amplification products were purified and sequenced. The PFGE patterns revealed the high level of heterogeneity of S. uberis, with 26 types of PFGE patterns. A high prevalence of scpA, fbp, lbp, lmb and acdA genes (100%-97%) was detected, whereas 79.41% of S. uberis harbored the sua gene. A high degree of similarity in the nucleotide and amino acid sequences of the 6 genes was observed. Our results showed that all genes are conserved and are present in most S. uberis isolates despite the wide clonal heterogeneity detected. This is the first study reporting an analysis of prevalence, and nucleotides and amino acids sequences of the potential adherence genes scpA, acdA, fbp, lbp, and lmb from S. uberis strains versus reported GenBank sequences, S. uberis 0140J and S. uberis NZ01.