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Peer-reviewed veterinary case report

Genotyping Protocol for the Alpha-1 Antitrypsin (PiZ) Mouse Model.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2017
Authors:
Gruntman, Alisha M
Affiliation:
University of Massachusetts Medical School · United States
Species:
rodent

Abstract

The most common alpha-1 antitrypsin (AAT) mutant variant is a missense mutation (E342K), commonly referred to as PiZ. A transgenic mouse model exists that expresses the mutant human PiZ AAT gene. This protocol outlines the procedure used to extract DNA from and genotype AAT transgenic (PiZ) mice.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/28752446/