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Peer-reviewed veterinary case report

Testing for infectious laryngotracheitis in chickens

By Kanabagatte Basavarajappa, Mallikarjuna et al.·Published in Journal of clinical microbiology·2015·Virginia-Maryland Regional College of Veterinary Medicine, United States·View original on PubMed

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Original publication title: Glycoprotein-based enzyme-linked immunosorbent assays for serodiagnosis of infectious laryngotracheitis.

Species:
bird

Plain-English summary

Researchers have created new tests to help detect antibodies against the infectious laryngotracheitis virus (ILTV) in chickens. These tests, known as B- and D-ELISAs, were found to be better at identifying these antibodies than a standard commercial test. Additionally, the D-ELISA was effective in showing when chickens developed antibodies after being vaccinated with a special vaccine that uses a modified Newcastle disease virus. Overall, these new tests could improve how we diagnose and monitor ILTV in chickens.

Abstract

For detection of infectious laryngotracheitis virus (ILTV) antibody, glycoprotein B-, C-, and D-based enzyme-linked immunosorbent assays (B-, C-, and D-ELISAs, respectively) were developed. The B- and D-ELISAs showed enhanced detection of anti-ILTV antibodies in infected chickens compared to that of the commercial ELISA. Furthermore, the D-ELISA was efficient in detecting seroconversion with vectored vaccine, using recombinant Newcastle disease virus (rNDV) expressing glycoprotein D (gD) as the vaccine vector.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25694519/