Peer-reviewed veterinary case report
Glycoprotein-based enzyme-linked immunosorbent assays for serodiagnosis of infectious laryngotracheitis.
- Journal:
- Journal of clinical microbiology
- Year:
- 2015
- Authors:
- Kanabagatte Basavarajappa, Mallikarjuna et al.
- Affiliation:
- Virginia-Maryland Regional College of Veterinary Medicine · United States
Plain-English summary
Researchers have created new tests to help detect antibodies against the infectious laryngotracheitis virus (ILTV) in chickens. These tests, known as B- and D-ELISAs, were found to be better at identifying these antibodies than a standard commercial test. Additionally, the D-ELISA was effective in showing when chickens developed antibodies after being vaccinated with a special vaccine that uses a modified Newcastle disease virus. Overall, these new tests could improve how we diagnose and monitor ILTV in chickens.
Abstract
For detection of infectious laryngotracheitis virus (ILTV) antibody, glycoprotein B-, C-, and D-based enzyme-linked immunosorbent assays (B-, C-, and D-ELISAs, respectively) were developed. The B- and D-ELISAs showed enhanced detection of anti-ILTV antibodies in infected chickens compared to that of the commercial ELISA. Furthermore, the D-ELISA was efficient in detecting seroconversion with vectored vaccine, using recombinant Newcastle disease virus (rNDV) expressing glycoprotein D (gD) as the vaccine vector.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25694519/