HBXN2020 alleviatesTyphimurium infection in mice by improving intestinal barrier integrity and reducing inflammation.

Abstract

is a species ofthat has been widely investigated because of its broad-spectrum antimicrobial activity. However, most studies onhave focused on the biocontrol of plant diseases, with few reports on antagonizingTyphimurium infections. In this investigation, it was discovered thatHBXN2020, which was isolated from healthy black pigs, possessed strong anti-stress and broad-spectrum antibacterial activity. Importantly,HBXN2020 did not cause any adverse side effects in mice when administered at various doses (1×10, 1×10, and 1×10CFU) for 14 days. SupplementingHBXN2020 spores, either as a curative or preventive measure, dramatically reduced the levels ofTyphimurium ATCC14028 in the mice's feces, ileum, cecum, and colon, as well as the disease activity index (DAI), in a model of infection caused by this pathogen in mice. Additionally, supplementingHBXN2020 spores significantly regulated cytokine levels (,,, and) and maintained the expression of tight junction proteins and mucin protein. Most importantly, addingHBXN2020 spores to the colonic microbiota improved its stability and increased the amount of beneficial bacteria (and). All together,HBXN2020 can improve intestinal microbiota stability and barrier integrity and reduce inflammation to help treat infection byTyphimurium.