Hematocrit estimates comparing centrifugation to a point-of-care method in beef cattle living at high altitude.

Abstract

BACKGROUND: The hematocrit (Hct) or packed cell volume (PCV) reflects the blood volume occupied by red blood cells. The development of point-of-care (PoC) instruments can accelerate the ease of measuring Hct/PCV compared with traditional capillary centrifugation (TCC) methods. However, no studies have compared Hct/PCV levels in cattle at high elevation with other measurement methods. OBJECTIVES: In this study, we aimed to compare methods to estimate Hcts/PCVs of rangeland cattle at high elevation. We specifically wanted to determine if Hct/PCV levels measured with a commercial PoC instrument (i-Stat with CHEM8+ cartridges [PoC]) were comparable to Hct/PCV levels measured with traditional laboratory methods. METHODS: We assessed the Hct/PCV of 94 mature beef cattle (black Angus; Bos taurus) at ~2195 m above sea level using paired analyses of the PoCand TCC methods from each animal. We used paired samples t-tests to compare mean Hct/PCVs. Correlation analyses relative to the line of identity and Passing-Bablok regression were used to assess systematic and proportional differences, respectively, and Bland-Altman plots were used to assess agreement between the two methods. RESULTS: The PoCestimated a Hct of 28.2% ± 0.7% (SE), which was lower than the TCC estimated PCV of 39.2% ± 0.5%. The Bland-Altman plot revealed poor agreement between the two methods in addition to a -11% bias for the PoC. The Passing-Bablok regression revealed both systematic and proportional bias between the two methods. CONCLUSIONS: Point-of-care blood instruments were not comparable to TCC methods for quantifying Hct/PCVs of cattle living at high elevations.