HFY06 attenuates D-galactose-induced oxidative stress and inflammation in male Kunming mice.

Abstract

There has been considerable research on oxidative stress and inflammation, and their relationship with degenerative diseases. This study investigated the effect ofHFY06 on aging mice induced by D-galactose. The results showed thatHFY06 inhibited the atrophy of the brain, kidneys, liver, and spleen, increased serum SOD, GSH, CAT, and MDA, and decreased IL-6, IL-1β, TNF-α, and IFN-γ. Quantitative PCR showed thatHFY06 upregulated the expression of Nrf2, γ-GCS, NOS1, NOS3, SOD1, SOD2, and CAT in the liver and brain tissues, but decreased the expression of NOS2. Western blot analysis showed thatHFY06 effectively upregulated the protein expression of SOD1, SOD2, and CAT in the livers and brains of mice. These results suggest thatHFY06 can effectively alleviate D-galactose-induced aging in mice, and may activate the Nrf2 signaling pathway and increase the levels of downstream regulatory inflammatory factors and antioxidant enzymes. In conclusion, consumption ofHFY06 may prevent aging or reduce oxidative stress.