High-purity soybean insoluble dietary fiber -microencapsulated Clostridium typhimurium: Intervention mechanisms in alleviating intestinal Dysbiosis.

Abstract

The present study utilized high-purity soybean insoluble dietary fiber (HPIDF) as the primary wall material to encapsulate Clostridium typhimurium, thereby establishing a novel microcapsule system (HPIDF-MIC). A mouse model of intestinal microbiota dysbiosis was successfully established through cefuroxime induction. Multi-modal analysis revealed that the ingestion of HPIDF-MIC alleviated weight loss and colonic oedema, with a 53 % reduction in the colonic injury index when compared to the model group. In addition, inflammation and oxidative stress were inhibited, total SCFAs were restored to 1503.54 μg/g (approaching the control group's 1578.71 μg/g), and intestinal microbiota diversity was restored, thus achieving multidimensional intestinal protective effects. The efficacy of the intervention was found to significantly outperform that of single-component (HPIDF) and traditional probiotic interventions. The present study systematically investigated the intervention effects and mechanistic basis of HPIDF-MIC on intestinal homeostasis, providing an efficient and safe candidate solution for the treatment of intestinal diseases. This study not only demonstrates the potential of HPIDF as a functional food ingredient in maintaining gut health but also provides mechanistic insights for guiding the development of microbiome-targeted nutritional supplements.