Peer-reviewed veterinary case report
High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates.
- Journal:
- Journal of microbiological methods
- Year:
- 2012
- Authors:
- Vorimore, Fabien et al.
- Affiliation:
- Bacterial Zoonoses Unit · France
Abstract
We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22652433/