Peer-reviewed veterinary case report
High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type".
- Journal:
- Journal of microbiological methods
- Year:
- 2012
- Authors:
- Douarre, Pierre E et al.
- Affiliation:
- Cork Institute of Technology
Abstract
In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22033498/