Histomorphometry of marrow megakaryocytes in experimental uraemia in rats.

Abstract

Uraemic patients frequently demonstrate tendencies towards life-threatening bleeding. Reduced platelet counts and their functional immaturity seem to be caused, among other things, by disorders in the system of marrow megakaryocytes. The aim of the study was a histomorphometric evaluation of marrow megakaryocytes in the course of experimental uraemia in rats. Uraemia was induced by means of right nephrectomy and a partial removal of the left kidney cortex in rats. Morphometric analysis was conducted, using the Microlmage program set. The number of MK, MK area, N/C, CDMK, CDNMK and MK cluster formation were analysed. Uraemic rats showed a reduction in the MK count and their area and an increase in the N/C ratio, CDMK, CDNMK and in the incidence of MK clusters. The results indicate that platelet disorders, observed in uraemia, can also be conditioned by disturbed maturation of MK.