Peer-reviewed veterinary case report
Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites.
- Year:
- 2023
- Authors:
- Ibarra-Meneses AV et al.
- Affiliation:
- Department of Pathology and Microbiology · Canada
Abstract
Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from cultured parasites. We detail a user-friendly bioinformatics pipeline for sequence analysis and visualization of CNV analysis and ploidy changes. For complete details on the use and execution of this protocol, please refer to Douanne et al. (2022).<sup>1</sup>.
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Search related cases →Original publication: https://europepmc.org/article/MED/37087735