Identification and complete genome analysis of kobuvirus in faecal samples of European roller (Coracias garrulus): for the first time in a bird.

Abstract

The genus Kobuvirus (Picornaviridae) consists of three species, Aichivirus A (e.g., Aichi virus, which infects humans), Aichivirus B and Aichivirus C. Kobuvirus have not been detected in non-mammal species including birds. In this study, a novel kobuvirus was identified in 3 (17 %) out of 18 faecal samples collected from European rollers (Coracias garrulus) in Hungary. The complete genome sequence of strain SZAL6-KoV/2011/HUN (KJ934637), which was determined using a novel 5'/3' RACE method (dsRNA-RACE) involving a double-stranded (ds)RNA intermediate, has a type-V IRES at the 5' end and a cis-acting element (CRE) in the 3C gene and encodes L and 2A(H-box/NC) proteins, but it does not contain the sequence forming a "barbell-like" secondary RNA structure in the 3'UTR. SZAL6-KoV/2011/HUN has 72 %, 73 %, and 81 % amino acid sequence identity to the P1, P2, and P3 protein, respectively, of Aichi virus. Evolutionary analysis showed that SZAL6-KoV/2011/HUN shares a common ancestor with other kobuviruses but belongs to a more ancient lineage in the species Aichivirus A. Investigation of the known kobuviruses in different animals and discovery of novel kobuviruses in potential host species helps to clarify the evolutionary connection and zoonotic potential of kobuviruses.