Peer-reviewed veterinary case report
How molecular tests identify canine parvovirus types
By Vasavi, Koppu et al.·Published in Current Journal of Applied Science and Technology·2021·View original on Crossref →
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Original publication title: Identification and Typing of Canine Parvovirus Using Molecular Techniques
- Species:
- dog
Plain-English summary
A group of dogs suspected of having parvovirus were tested using rectal swabs to identify the virus responsible for their symptoms, which can include severe vomiting and diarrhea. The tests showed that a significant number of these dogs were infected, with the most common type being CPV 2a. Alarmingly, some vaccinated dogs also tested positive for the virus, indicating that the vaccine may not fully protect against all strains. This research highlights the importance of monitoring parvovirus infections in dogs, even among those that have been vaccinated.
People also search for: dog vomiting diarrhea parvovirus · canine parvovirus vaccine effectiveness · symptoms of parvovirus in dogs
Abstract
Aim: Canine parvovirus 2, the causative agent of acute hemorrhagic enteritis in dogs, is one of the most important pathogenic viruses. It causes a highly contagious and often fatal disease. The disease condition is complicated further due to emergence of a number of variants namely CPV-2a, CPV-2b, CPV-2c, new CPV 2a, new CPV 2b over the years and involvement of domestic and wild canines. The virus is shed in large numbers in the feces of infected dog and upto 7 to 10 days post-infection, therefore, the present study was designed to detect CPV and to identify the prevailing antigenic types of CPV using molecular techniques from rectal swabs of affected dogs.
 Methods: The rectal swabs were collected from dogs suspected of Canine Parvovirus and subjected to PCR, Nested PCR and Realtime PCR for identification and typing of CPV in infected dogs.
 Results: From the study it was found that the per cent positivity was high in dogs and was found to be 50% and 89% by PCR and nested polymerase reaction respectively when considered in suspected dogs. The most prevailing antigenic type as detected by Real time PCR was found to be CPV 2a.
 Conclusions: The study indicated the animals vaccinated for CPV were also found positive for the disease. This study helps to detect percent positivity of CPV in dogs and also is important to identify the prevailing antigenic types of CPV in the region.
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Search related cases →Original publication on Crossref: https://doi.org/10.9734/cjast/2021/v40i1931457