Peer-reviewed veterinary case report
New Babesia gibsoni gene helps detect infection in dogs
By Jia, Honglin et al.·Published in The American journal of tropical medicine and hygiene·2006·Obihiro University of Agriculture and Veterinary Medicine, Japan·View original on PubMed →
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Original publication title: Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis.
- Species:
- dog
Plain-English summary
A dog infected with Babesia gibsoni, a parasite that can cause serious health issues, was used in research to help develop a better blood test for diagnosing this infection. Scientists discovered a new protein called BgSA1 that can be detected in the blood of infected dogs. They found that a new test using BgSA1 was more effective than previous tests at identifying the infection. This means that BgSA1 could help create a more accurate diagnostic test for veterinarians to use when checking for Babesia gibsoni in dogs.
People also search for: dog Babesia gibsoni symptoms · dog blood test for Babesia · Babesia gibsoni treatment in dogs
Abstract
Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These results indicated that BgSA1 could be a useful target for the development of a diagnostic test for the detection of specific antibodies and circulating antigens.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/17123975/