Peer-reviewed veterinary case report
Bacteria linked to ear infections in dogs identified by DNA sequencing
By Saengchoowong, Suthat et al.·Published in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]·2023·Princess Srisavangavadhana College of Medicine·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Identification of bacteria associated with canine otitis externa based on 16S rDNA high-throughput sequencing.
- Species:
- dog
Plain-English summary
A group of dogs with ear infections (otitis externa) was studied to identify the types of bacteria present in their ears. The researchers found that dogs with healthy ears had a greater variety of bacteria compared to those with infections. Common bacteria found in the infected dogs included Corynebacterium, Pseudomonas, Staphylococcus, and Proteus. Some new types of bacteria that could be causing infections were also discovered. This information can help veterinarians better understand and treat ear infections in dogs.
People also search for: dog ear infection bacteria · why does my dog have ear problems · treatment for dog otitis externa
Abstract
Bacteria are regarded as predisposing and perpetuating factors causing otitis externa (OE), whereas auricular anatomy is a predisposing factor. This study aims to investigate bacterial populations in the external auditory canals of healthy dogs and dogs with OE. Four categories of ear swabs included healthy erect-ear dogs, erect-ear dogs with OE, healthy pendulous-ear dogs and pendulous-ear dogs with OE. After bacterial DNA extraction, 16S rDNAs were amplified using specific primers within a V3/V4 region. Following DNA library construction, high-throughput sequencing was performed on MiSeq (Illumina). CLC Microbial Genomics Module was used to determine the rarefaction curve, bacterial classification, relative abundance, richness and diversity index. The results demonstrated that healthy dogs had higher bacterial richness and diversity than the dogs with OE. Comparable with culture-dependent methods described previously, this study revealed predominant Corynebacterium spp., Pseudomonas spp., Staphylococcus spp., and Proteus spp. in OE cases. Furthermore, high-throughput sequencing might disclose some potential emerging pathogens including Tissierella spp., Acinetobacter spp., and Achromobacter spp., which have not been reported in previous canine OE cases. Nevertheless, larger sample sizes are further required for an extensive evidence-based investigation.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/37889464/