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Peer-reviewed veterinary case report

Identification of Bartonella henselae in an aborted equine fetus.

Journal:
Veterinary pathology
Year:
2009
Authors:
Johnson, R et al.
Affiliation:
Purdue University · United States
Species:
horse

Plain-English summary

This report discusses a case where a horse fetus was aborted due to an infection with a bacterium called Bartonella henselae, which is known to cause various diseases in humans and animals. The fetus showed signs of tissue damage and inflammation, and tests confirmed the presence of this bacterium in its tissues. Researchers used several methods to identify the bacteria, including genetic testing, and found that it closely matched known strains of B. henselae. Despite looking for other common causes of abortion in horses, they found no evidence of those. The conclusion was that the infection with B. henselae was responsible for the abortion of this foal.

Abstract

This report describes the characterization of a Bartonella henselae abortion in an equine fetus by gross, histologic, immunohistochemical, ultrastructural, and molecular methods. Bartonella henselae can cause cat scratch disease, bacillary angiomatosis, bacillary peliosis, and endocarditis in humans and other animals. The bacterium has been isolated from several mammalian species but only recently from equids; however, it has not been linked to abortion in equids. An aborted equine fetus exhibited necrosis and vasculitis in multiple tissues, with intralesional Gram-negative short-to-spirillar bacteria. Nucleotide sequence analysis of the bacterial 16S rRNA gene amplified from the DNA extracted from fetal tissues revealed 99.9% homology to that of B. henselae. The presence of B. henselae in the fetal tissues was further confirmed by polymerase chain reaction amplification and nucleotide sequence analysis of other Bartonella species-specific genes. Microorganisms were immunohistochemically labeled with a monoclonal antibody to B. henselae and were ultrastructurally characterized. Attempts to detect known causative agents of equine abortion were unsuccessful. Given the severity of vasculitis and the presence of intralesional bacteria, we concluded that B. henselae infection caused the abortion of this foal.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/19261640/