Peer-reviewed veterinary case report
MicroRNA changes in skin of atopic beagles with allergy
By Santoro, Domenico et al.·Published in Immunogenetics·2020·Department of Small Animal Clinical Sciences, United States·View original on PubMed →
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Original publication title: Identification of differentially expressed microRNAs in the skin of experimentally sensitized naturally affected atopic beagles by next-generation sequencing.
- Species:
- dog
Plain-English summary
A group of beagles with atopic dermatitis (a common skin allergy) had skin samples taken to study the genetic changes associated with their condition. Researchers compared the skin of these affected dogs to healthy beagles and found significant differences in the levels of certain microRNAs, which are small molecules that can indicate inflammation. They discovered that many microRNAs were less active in the skin of the atopic dogs, suggesting a link between these changes and the skin problems caused by allergies. This research is a first step in understanding how microRNAs might play a role in managing skin allergies in dogs.
People also search for: beagle skin allergy treatment · atopic dermatitis in dogs · microRNA skin problems in dogs
Abstract
Canine atopic dermatitis (AD) is a very common inflammatory skin disease, but limited data are available on the genetic characterization (somatic mutations, microarrays, and genome-wide association study (GWAS)) of skin lesions in affected dogs. microRNAs are good biomarkers in inflammatory and neoplastic diseases in people. The aim of this study was to evaluate microRNA expression in the skin of atopic beagles, before and after exposure to Dermatophagoides farinae. Four atopic and four unrelated age-matched healthy beagle dogs were enrolled. Total RNA was extracted from flash-frozen skin biopsies of healthy and atopic dogs. For the atopic dogs, skin biopsies were taken from non-lesional (day 0) and lesional skin (day 28 of weekly environmental challenge with Dermatophagoides farinae). Small RNA libraries were constructed and sequenced. The microRNA sequences were aligned to CanFam3.1 genome. Differential expressed microRNAs were selected on the basis of fold-change and statistical significance (fold-change ≥ 1.5 and p ≤ 0.05 as thresholds. A total of 277 microRNAs were sequenced. One hundred and twenty-one differentially regulated microRNAs were identified between non-lesional and healthy skin. Among these, two were increased amount and 119 were decreased amount. A total of 45 differentially regulated microRNAs between lesional and healthy skin were identified, 44 were decreased amount and one was increased amount. Finally, only two increased amount microRNAs were present in lesional skin when compared with that of non-lesional skin. This is the first study in which dysregulation of microRNAs has been associated with lesional and non-lesional canine AD. Larger studies are needed to understand the role of microRNA in canine AD.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/32219493/