Peer-reviewed veterinary case report
Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses.
- Journal:
- Veterinary microbiology
- Year:
- 2014
- Authors:
- Bartley, Kathryn et al.
- Affiliation:
- Moredun Research Institute · United Kingdom
Abstract
Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25091530/