Peer-reviewed veterinary case report
Key proteins identified for diagnosing leptospirosis in dogs
By Thomé, Sabrina et al.·Published in BMC veterinary research·2014·Department of Microbiology and Parasitology, Brazil·View original on PubMed →
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Original publication title: Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA).
- Species:
- dog
Plain-English summary
A group of 109 dogs suspected of having leptospirosis, a serious bacterial infection, were tested to find better ways to diagnose the disease. Researchers looked for specific proteins from the bacteria that could be detected in the dogs' blood. They found that certain proteins, particularly LigB and LipL32, were effective at identifying infected dogs, showing high accuracy in tests. This study suggests that these proteins could help develop improved tests for diagnosing leptospirosis in dogs, which could lead to quicker and more accurate treatment.
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Abstract
BACKGROUND: The microscopic agglutination test (MAT), the standard method for serological diagnosis of leptospirosis, may present limitations regarding its sensitivity. Current studies suggest that Leptospira immunoglobulin-like (Lig) proteins and LipL32 are of particular interest as serodiagnostic markers since they are present only in pathogenic species of the Leptospira genus. The purpose of this study was to identify leptospiral immunodominant proteins that are recognized by canine sera from diseased dogs. RESULTS: A total of 109 dogs were studied, including seroreactive dogs (MAT ≥800) and dogs with no seroreactivity detectable by MAT. Eight recombinant fragments (31-70 kDa) of pathogenic Leptospira were tested for their use as diagnostic markers for canine leptospirosis using the Multi-antigen Print Immunoassay (MAPIA) platform: LigB [582-947aa] from L. interrogans serovar Pomona, L. interrogans serovar Copenhageni and L. kirschneri serovar Gryppotyphosa, LigB [131-649aa] from L. interrogans serovar Copenhageni, L. interrogans serovar Canicola and L. kirschneri serovar Gryppotyphosa, LigA [625-1224aa] L. interrogans serovar Copenhageni and LipL32 from L. interrogans serovar Copenhageni. The data were analyzed and ROC curves were generated. Altogether, LigB [131-649aa] L. interrogans Canicola, LigB [131-649aa] L. kirschneri Gryppotyphosa and LipL32 L. interrogans Copenhageni showed best accuracy (AUC = 0.826 to 0.869), with 70% specificity and sensitivity ranging from 89% to 95%. CONCLUSIONS: These results reinforce their potential as diagnostic candidates for the development of new methods for the serological diagnosis of canine leptospirosis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25466383/